Hello,
I want to generate bone marrow derived DCs (BMDCs) and want to use supernatant of a GM-CSF producing cell line for the GM-CSF source.
Does someone know a cell line (mouse) which is producing GM-CSF in the supernatant and where I can get them from?
I used once X6310 cells, which produced GM-CSF, but I couldn't find a source where to buy them.
Thank you,
Dominic
Hey,
I used to use conditioned media from the J558 cell line in order to generate BMDCs.
Regards,
Daniel
Hi Daniel,
Thank you very much. I contacted my old lab and they have a cell line called "X63Ag8-653 cells" and they will give me an aliquot.
Best wishes,
Dominic
Hi Dominic,
I know that there´s another cell line: BHK-HM5, that produces mGM-CSF, but I haven´t been able to find it from a commercial source. I could only find the BHK cell line (in ATCC, BHK-21 [C-13]) and I guess this one must be transfected first with a plasmid to use it as a GM-CSF source.... Here´s a reference link: Article Current Protocols in Immunology
Regards,
Ana
Hello Dominic,
ABM (www.abmgood.com) has a large collection of cell lines available. You can ask them if they have this cell line.
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