Ana Ramon Vazquez

2 Questions 3 Answers 0 Followers

Questions related from Ana Ramon Vazquez

Can I concentrate supernatants for ELISA tests using a centrifugal filter device?

The supernatants are from thioglycollate elicited peritoneal macrophages treated with LPS, or LPS and ATP.

04 April 2013 6,582 3 View

How can I calculate concentrations for cytokines from my data obtained in an ELISA assay if my plate reader doesn't have "wavelength correction"?

I have used the substrate "ABTS" (Sigma) which needs a "wavelenght correction set at 650nm", as specified on the Data sheet. I think the plate reader (BioRad) is quite old and I haven´t been able...

04 April 2013 2,399 1 View