Does anyone know how to extract proteins from swabs?

I would like to run ELISA to check cathelicidin level, but I don't have a protocol to extract this protein from vaginal swabs. Can anyone help me?

04 May 2014 1,562 2 View

ELISA kit for anti-MICA?

I would like to determine the level of antibodies against MICA in human plasma/serum using ELISA, does anybody know if there is a commercially available kit to do this kind of analysis?

01 February 2013 7,152 3 View

Blank correction in ELISA.

31 December 2012 4,172 9 View

Absorbance limits

Is it possible to say which is the minimum and maximum absorbance limit in ELISA tests? Since linearity of transmittance breaks down at higher concentration because of sample saturation and...

31 December 2012 587 7 View

Low concentration of the protein in the samples - ELISA kit

I have a problem. Concentrations of my unknown samples are very low, their absorbances are almost at the level of blank, is it possible to detect my protein anyway? [urine probes, L-FABP ELISA...

31 December 2012 7,593 17 View

Curve fitting in ELISA

1. Can I assume that it is better to use 5 parameter logistic (5PL) fit than the 4PL for sigmoidal shape curves? (4PL assumes symmetry of the standard curve around the inflection point where the...

31 December 2012 7,270 11 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View