I would like to run ELISA to check cathelicidin level, but I don't have a protocol to extract this protein from vaginal swabs. Can anyone help me?
Thank you all for answering.
I'm going to try protocol decribed in paper PMC3790320 (Marcia M. Hobbs et all.)
"Vaginal specimens were collected on cotton-tipped swabs. Immediately after collection, swabs were air-dried, stored in screw-capped tubes. For recovery of vaginal secretions, each swab was placed into 3.0 mL phosphate-buffered saline, incubated at room temperature for 15 – 30 min, agitated and pressed against the side of the tube to elute the sample. Vaginal specimens were centrifuged at 250 × g for 10 min, and the supernatants were removed from cell pellets and stored at −80°C until testing."
(just in case someone else needs it)
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