7 Questions 11 Answers 0 Followers
Questions related from Hanna Jerczynska
I would like to run ELISA to check cathelicidin level, but I don't have a protocol to extract this protein from vaginal swabs. Can anyone help me?
05 May 2014 1,616 2 View
Did anybody determine protein’s glycation in human platelets using commercially available ELISA kit? If yes, which was the kit?
02 February 2013 9,763 1 View
I would like to determine the level of antibodies against MICA in human plasma/serum using ELISA, does anybody know if there is a commercially available kit to do this kind of analysis?
02 February 2013 7,190 3 View
Is it possible to say which is the minimum and maximum absorbance limit in ELISA tests? Since linearity of transmittance breaks down at higher concentration because of sample saturation and...
01 January 2013 753 7 View
Recently I got a little bit confused. Generally, I was always doing blank correction to all my data in ELISA tests, but I started to wonder if I’m right in doing this because, in the case of...
01 January 2013 4,319 9 View
I have a problem. Concentrations of my unknown samples are very low, their absorbances are almost at the level of blank, is it possible to detect my protein anyway? [urine probes, L-FABP ELISA...
01 January 2013 7,639 17 View
1. Can I assume that it is better to use 5 parameter logistic (5PL) fit than the 4PL for sigmoidal shape curves? (4PL assumes symmetry of the standard curve around the inflection point where the...
01 January 2013 7,578 11 View