Do you have any suggestions for me to modify my LS-DYNA model?

Hi, I use LS-DYNA to simulate the steel composite shear wall now. I use MAT_084/085 to simulate concrete, MAT_159 to simulate steel plates, and MAT_024 to simulate studs. The concrete section...

24 January 2021 3,863 1 View

Why there is a band around 25Kd of caspase3 in both undifferentiated and differentiated PC12 cells?

Dear all, When I detect the protein expression of caspase3 in PC12 cells, there is always a band around 25Kd. According to literature, the pro-caspase3 should be 35Kd and the cleaved caspase 3...

21 September 2020 8,577 2 View

How to claim back your publication?

My publications have been wrongly claimed by other ppl and I could not claim it back. Browsed through all the pages/tabs, but still could not find where to make the complain. Anyone knows how to...

19 August 2018 7,538 13 View

Does anyone have information about plane failure on rock slope?

07 April 2016 8,054 3 View

What is the NRTL model in chemical engineering?

I'm wondering what is the NRTL model and its usages. I wanna know about NRTL HOC, as well. Please let me know. Thanks.

27 June 2015 5,427 3 View

How to get the standard redox potential of CO2 + 6H+ + 6e- -CH3OH + H2O -0.38 V vs NHE at pH7?

08 February 2015 2,269 3 View

Is it possible to measurement Ca2+ concentration in neurons using a microplate reader?

I want to use fluo-3 to detect the intracellular Ca2+ and I used spectramax M5. Can I use serum-free medium as the buffer?

18 December 2014 4,361 3 View

How to set the parameters for valence band XPS checking?

Normally, we only check the survey XPS and high resoulution XPS of different elements. How to check the valence band XPS? can we check the XPS VB by seting the survey scan range from 20 eV to -5...

01 January 1970 9,086 7 View

How to respond to biased reviews

It has been almost 15 years since my first submission of a manuscript for journal publication. Some of my recent experience, grateful and furious, makes me decide to write such a post. After...

01 January 1970 2,213 6 View

How can Virtual Reality be applied for pediatric imaging and radiology?

Virtual reality is an emerging and essential technology in computer science. It is also useful for medical cares such as surgical simulation and understanding viruses. But how could the virtual...

01 January 1970 6,274 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View