Hello,
I am looking at glucose detection assays based on calorimetry. Due to the volume of samples (around 40 conditions per cell line and 6 cell lines), kits are not feasible for us. Has anybody created an in-house assay that they are willing to share?
Thanks!
glucose oxidase ... Dextrostix®,
Benedict developed a copper reagent for urine glucose, which was used, with some modifications, for more than 50 years (1). The cumbersome methodology of heating became more convenient in 1945 with the development of Clinitest (Ames, Elkhart, IN), which featured a modified copper reagent tablet. Glucose was oxidized, and the amount of glycosuria was proportional to the color of the heated solution. In 1965, Ames developed the first blood glucose test strip, the Dextrostix, using glucose oxidase. A large drop of blood was placed on the strip and, after 60 seconds, was washed away. The generated color was then compared to a chart on the bottle for a semi-quantitative assessment of blood glucose
best wishes!
Hi Macarena Lucia Fernandez Carro ,
I use DNS assay (Dinitrosalycilic Assay) to detect reducing sugars, especially glucose that is released by the cellulolytic activity of microorganisms on cellulosic materials. It is a renowned method that you can find in much literature. Please read about it and check whether it will be any help for your purpose. Following are the details of the original article for this assay
1. Use of dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
G. L. Miller
Analytical Chemistry 1959 31 (3), 426-428
DOI: 10.1021/ac60147a030
thanks! I am looking at using GOx and Peroxidase method for colorimetric cuanqitfication
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