Hi,
Does anyone know about a public transcriptomic dataset (RNAseq, microarray) of Arabidopsis (or other plants) treated with Staurosporine? I checked in GEO or SRA, but I couldn't find any.
Thanks!
Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...
11 July 2024 6,847 0 View
Hello! I´m developing a sandwich ELISA. My assay recognizes protein denaturalized very well, but there isn´t recognition when it is in its native form. So maybe if I heat the sample I could detect...
18 April 2024 5,671 3 View
Hello all, I present here a problem to see if someone has encountered it and can provide some clarity. We are trying to isolate neutrophils from patient blood in tubes EDTA. We are following the...
10 March 2024 4,155 3 View
Hello everyone, I am starting to grow the SCTi003-A iPSC line for the first time and I am facing some drawbacks. Due to some problems with the first pass, I waited a bit longer than the 7-8 days...
22 February 2024 8,051 2 View
I have been performing titrations of a known concentration of hydrogen peroxide using 0.01N potassium permanganate and consistently requiring more potassium permanganate than what is estimated to...
07 February 2024 445 3 View
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06 February 2024 1,523 6 View
I'm running XF T Cell persistence assay in a mini Seahorse. I used to not have problems until I start getting some negatives values in OCR measurements, and also errors with both background wells,...
12 January 2024 4,454 0 View
Good morning, colleague My name is Víctor Hugo Fernández Bedoya, and I am a professor at the National University of San Marcos (Universidad Nacional Mayor de San Marcos), located in Lima, Peru. I...
08 January 2024 1,651 0 View
Hello everyone, I need advice on improving the quality of my coronal slices obtained from PFA-fixed mouse brains using a cryostat. As You can see in the attached image, slices are horrible! Any...
04 January 2024 6,070 2 View
Hi, I'm working with viral cultures, specifically with Dengue virus serotype 2. I need to quantify it by a plaque assay or TCID50. Does anyone have a protocol or has worked with this serotype...
10 December 2023 2,363 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,881 2 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,747 4 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,300 7 View
I have a dataset consisting of json files. i tried to upload a zip or tar of it but the system tells me that the file format is not accepted... br
25 July 2024 1,315 3 View
Hello, I'm working on medical project and I would need synchronized angiography with ECG? Does anyone know if some open source dataset of this kind exist? Regards, Bruno
25 July 2024 2,213 2 View
I'm working on a project that involves analyzing a new dataset, and I'm at the stage of selecting the most appropriate machine learning algorithm. The dataset consists of both numerical and...
22 July 2024 6,095 7 View
Hi, I wanna to implement evolutionary algorithms in ryu sdn controller in mininet, i have some challenges, how i can run the big scale topo with one sdn contoller??? and another question is to...
21 July 2024 244 2 View
I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...
20 July 2024 1,338 2 View
I am currently working on a mendelian randomization study, and I have downloaded the datasets needed from the ieu opengwas project (mrcieu.ac.uk) in .vcf format. I do not have access to an R...
19 July 2024 2,342 5 View
Anomaly detection in scanned image data set
18 July 2024 3,576 3 View