Is there any difference between using TC-treated glass or polystyrene culture plates to grow intestinal organoids?
I would prefer to grow organoids on glass but I am wondering if there is any evidence that there is a difference.
Here I am facing this problem. I don't find any effect of this type of parameters. I am a beginner user of COMSOL.
23 February 2021 9,086 3 View
Does the absorption coefficient of the semiconductor changes with the change in the structure of the semiconductor? If you have a thin film and nanowire of the same semiconductor, will the...
23 February 2021 9,799 4 View
I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?
22 February 2021 215 2 View
As fractal dimension of a thin film surface implies the irregularity in the surface. Is it possible that the energy states of the electrons are also affected by the fractal dimension?
19 February 2021 5,509 3 View
I have remotely sensed data (image) that I want to use in the Neuro-Fuzzy Designer apps of MATLAB. Is it possible to use an image file in the Neuro-Fuzzy Designer app or only numeric data is...
17 February 2021 1,205 5 View
To determine % K2O of organic manures, flame photometric technique is used whereas in inrganic fertiliser for K2O determination STPB method is used. I am trying to find out any method of...
24 January 2021 4,714 3 View
Hello, I had been trying to model a portal frame wrapped with CFRP in ABAQUS. To tie the CFRP laminates with frame I need to use slave/master surface interaction. But whenever I try to model the...
19 January 2021 2,906 3 View
I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...
18 January 2021 3,578 1 View
These days I am noticing that the mixture of NaOH (NH) (10-12M) and N2SiO3 (NS) solution gets solidify after 24 hours. The ratio of mixture of NS to NH is 2. To mention, the same solutions that we...
18 January 2021 7,911 2 View
Respected sir/mam, I want to simulate the urban expansion using different time series LULC based on satellite image. please suggest me most suitable model for urban simulation. Thanks and regards.
17 January 2021 9,134 11 View
02 March 2021 3,060 3 View
28 February 2021 9,936 2 View
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines
24 February 2021 2,268 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
22 February 2021 9,932 11 View