when determining the total anthocyanin content using a SO2 bleaching protocol, the results are showing only the quantity of anthocyanins (not bonded to tannins)? And the same question for the tannins determined by absorbance measurement at 550 nm after the acid hydrolysis. Will I get results of tannins not bound to anthocyanins? Or can I speculate that some molecules will be bounded? Thanks!!
@Nair
The hydrolysis reaction isn't complete, so you have to push the hydrolysis to 100%, either by spending more time or using a good catalyst
By HPLC, we can determine anthocyanin and its derivatives and tannins separate
Anthocyanins (At) are present in different forms: free anthocyanins (Al) and anthocyanins combined with tannins (Ac), some of which are bleached by SO2 (TA), while the rest is unaffected (TAT). In conclusion, by bleaching with SO2 some of the anthocyanins bonded to tannins (TA) will respond.
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