Is there any databases of sequencing data for AD mouse models?

I am working with the NL-G-F and 5x FAD mice. Are there any sequencing databases publicly available to mine for brain or retina? Thanks!

20 January 2021 6,425 1 View

Does anyone have problems performing CRISPR KO in human primary T cells?

For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...

11 January 2021 6,016 3 View

What are the applications of remote sensing and GIS in transportation, what are the types of sensors you will require?

How do you use Remote sensing and GIS in transportation, what are the types of images you will require, what will be their wavelength, what type of sensors will you require?

20 December 2020 6,375 5 View

Non-biological recognition elements for bacterial sensors?

Hi, I am interested in non-biological recognition elements for bacterial sensors. I am aware of molecularly imprinted polymers but I do not seem to find many other alternatives. I know that many...

18 September 2020 5,894 3 View

Linear Mixed model analysis - assessing baseline covariate?

I am analysing treatment group differences at time 2 (two time points only) data on a psych measure outcome (with five covariates: age, gender, CVD, BMI and F2s), and I am interested in one of the...

25 August 2020 6,904 3 View

Autoclaving Powder Skimmed Milk?

Hello! Does anyone has a effective way to autoclave 10% skimmed milk without caramelization? I tried 121C for 15min (in the powder form) but I am struggling with the color which is undesirable....

30 June 2020 4,601 3 View

Technique to estimate the amount of nanoparticles in a coating?

Hi everyone, I am coating stainless steel with 10%PLA and Nanoparticles, and I am trying to find out techniques that I can use to characterize the coating surface aside from SEM and to quantify...

08 June 2020 2,293 7 View

How to inactivate SARS-Cov-2 in tissue for spatial transcriptomics?

We want to run 10x Genomic Visium spatial transcriptomics on lung tissue from COVID-19+ patients. How can we inactivate the virus so it's safe to work with the tissue at BSL2? The first step is...

28 April 2020 4,704 3 View

How can one perform spin coating of stainless steel 316L with a polylactide-chloroform solution?

I am trying to do for my thesis a uniform homogeneous coating with PLA (polylactic acid with 1.05 dl/ g viscosity) in Stainless steel 316L medical grade via spin coating, but I haven't been able...

07 April 2020 8,556 3 View

What is the mutation rate in the mtDNA of the Lepidoptera?

I want to determine the phylogenetic age of the members of the Lepidoptera (Sphingidae) based on mtDNA, using BEAST software; But I do not have a good indicator. Please what is mutation rate in...

28 March 2020 1,559 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View