Hi all,
I am in the midst of extracting my snail tissue samples via Omega BioTek's EZNA Mollusc specific DNA extraction kit and I am having some shearing/degradation.
I extracted two samples as a test run and they produced a nice, clean band but every sample after I have gotten varying levels of shearing. I have been informed that the DNA is fine for downstream application but this is more for my own sanity...I really don't see what I am doing differently between the extractions. Have I introduced a small amount of DNase contamination? There are vigorous vortexing steps necessary to the removing of contaminants but I did those for all extractions I have done, including the first two.
All the best!
Brenna