Genomic DNA requirement for genotyping using SNPType assays (Fluidigm)?

More Nguyen Ngoc Nam's questions See All

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Leonid Ouchanov

I'd say 260/230 ratio lower than 1.5 might be a problem, but you never know. I would just try a couple of samples. What is the concentration required for your assay? If you can dilute your DNA 1:2 to 1:3 you can avoid the problem of organic contaminants.

Nguyen Ngoc Nam

Thanks for your response.

I diluted my samples, the ratios were improved a little. I asked a person who is working with SNP Type assays. He told me that A260/280 is best in a range of 1.8-2.0, but not mention A260/230 ratio.