Genolist_Subtilist: why it is not accessible?

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Chip_dna enrichment

Could you explain experimentally what (or how) I should do exactly in the "DNA enrichment" step during Cromatin Immunoprecipitation (ChIP) method. In my experiments, I (think) obtained the DNA...

09 October 2011 2,370 2 View

DNA polyA tail Enzyme for T/A cloning

Hi, Do you have any suggestion about PolyA ADDING ENZYMEs to add polyA to the DNA fragments which obtained at the end of ChIP experiments. I need specific enzyme brand or product number to...

08 September 2011 769 4 View

Cloning and expression vector for Bacillus subtilis....

Hi, All... I am looking for a cloning and expression vector for Bacillus subtilis. Of course I have some choices.... But let me know your experiences.. Do you have any suggestion for both...

08 September 2011 8,100 2 View

Dissolving of Phenol...

Hi, I would like anyone to clear using of Phenol. I am about prepare Phenol:Chloroform:isoamylalcohol (25:24:1). But the Phenol I have is crystillazed form and I must dissolve it. As I...

07 August 2011 6,659 12 View

USING FORMALDEHYDE

I am about beginnig to chromotinimmunoprecipitation (ChIP) experiments and it is indicated that to fixation I must use FORMALDEHYDE. I want to your help espacially who is applying the ChIP or...

05 June 2011 5,504 6 View

ChIP Control for Bacteria

Hi, I am studying some different KIT/protocol for ChIP technics. They suggest to use or include positive or negative control for human, mouse or rat. But I research on bacteria and I need...

05 June 2011 1,649 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View