Cloning and expression vector for Bacillus subtilis....

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Genolist_Subtilist: why it is not accessible?

The web site http://genolist.pasteur.fr/SubtiList/ is one of the important servers for my studies. It was accessible for general usage. But I haven't been able to access to this server for a...

11 December 2013 5,859 3 View

Chip_dna enrichment

Could you explain experimentally what (or how) I should do exactly in the "DNA enrichment" step during Cromatin Immunoprecipitation (ChIP) method. In my experiments, I (think) obtained the DNA...

09 October 2011 2,370 2 View

DNA polyA tail Enzyme for T/A cloning

Hi, Do you have any suggestion about PolyA ADDING ENZYMEs to add polyA to the DNA fragments which obtained at the end of ChIP experiments. I need specific enzyme brand or product number to...

08 September 2011 769 4 View

Dissolving of Phenol...

Hi, I would like anyone to clear using of Phenol. I am about prepare Phenol:Chloroform:isoamylalcohol (25:24:1). But the Phenol I have is crystillazed form and I must dissolve it. As I...

07 August 2011 6,659 12 View

USING FORMALDEHYDE

I am about beginnig to chromotinimmunoprecipitation (ChIP) experiments and it is indicated that to fixation I must use FORMALDEHYDE. I want to your help espacially who is applying the ChIP or...

05 June 2011 5,504 6 View

ChIP Control for Bacteria

Hi, I am studying some different KIT/protocol for ChIP technics. They suggest to use or include positive or negative control for human, mouse or rat. But I research on bacteria and I need...

05 June 2011 1,649 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View