I am about beginnig to chromotinimmunoprecipitation (ChIP) experiments
and it is indicated that to fixation I must use FORMALDEHYDE.
I want to your help espacially who is applying the ChIP or ChIP-on-ChIP experiments..
As you know, formaldehyde is toxic compond. I need to know that how I can use it
during my studies and what I must consider when I use it in the lab safely...
Thanks a lot..
Use gloves and avoid exposure to the vapours (especially eyes- work in a fume hood when using the main bottle). Make a small aliquote (which would be enough for ChIP type experiments) and use it.
Hi, we are having a separated liquid trash for formaldehyd-containing solutions. You will use it in low concentration (1%(v/v) final concentration from a 37%(v/v) stock solution), so you can work with it under the hood, especially at the step when you work with the stock solution. As I said finally discard the first solution in a separated container.
paraformaldehyd, formaldeyd.... formaldehyd is more expensive, because it is form function(o=c-H).
Are you sure Jıa about it cost. I asked its cost to a company and as I know its cost is about 17 euro (37%, 500 ml Formaldehyde) ... What did you mean about "expensive"?...
Read the documents provided form the company producing the formaldehyde. A part of the information describes the safety issues.
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