7 Questions 11 Answers 0 Followers
Questions related from Kemal Avci
The web site http://genolist.pasteur.fr/SubtiList/ is one of the important servers for my studies. It was accessible for general usage. But I haven't been able to access to this server for a...
12 December 2013 5,892 3 View
Could you explain experimentally what (or how) I should do exactly in the "DNA enrichment" step during Cromatin Immunoprecipitation (ChIP) method. In my experiments, I (think) obtained the DNA...
10 October 2011 2,404 2 View
Hi, Do you have any suggestion about PolyA ADDING ENZYMEs to add polyA to the DNA fragments which obtained at the end of ChIP experiments. I need specific enzyme brand or product number to...
09 September 2011 808 4 View
Hi, All... I am looking for a cloning and expression vector for Bacillus subtilis. Of course I have some choices.... But let me know your experiences.. Do you have any suggestion for both...
09 September 2011 8,140 2 View
Hi, I would like anyone to clear using of Phenol. I am about prepare Phenol:Chloroform:isoamylalcohol (25:24:1). But the Phenol I have is crystillazed form and I must dissolve it. As I...
08 August 2011 6,699 12 View
Hi, I am studying some different KIT/protocol for ChIP technics. They suggest to use or include positive or negative control for human, mouse or rat. But I research on bacteria and I need...
06 June 2011 1,684 2 View
I am about beginnig to chromotinimmunoprecipitation (ChIP) experiments and it is indicated that to fixation I must use FORMALDEHYDE. I want to your help espacially who is applying the ChIP or...
06 June 2011 5,537 6 View
