I know it depends, but am still wondering, on average, how much antibody would be used per lateral flow assay.
Thanks
Water resources are limited and have been significantly reduced by pollution due to urbanization in recent decades. Therefore, water resource optimization only highlights the ways these resources...
27 July 2024 9,303 1 View
Dear Scholars How is it possible to define an algorithm for data augmentation and use a surrogate model? In particular, I want to use data farming to create artificial data instead of real data...
12 June 2024 2,484 4 View
i need sample size calculator for quasi study design
02 June 2024 9,822 2 View
hello drs can you help me finding new topics in orthodontic for PhD research, i preferred clinical topics specially using CBCT
30 April 2024 9,258 3 View
In an interventional study, is there a criterion for choosing a secondary outcome or a second dependent variable? Should these two variables in the study of medical sciences and especially...
26 April 2024 9,695 1 View
I have been sent e.coli strains on filter paper and after 10 days I have not received them yet. I am wondering how long would the bacteria survive. Thank you in advance
21 April 2024 881 0 View
I performed genotyping analysis. Some samples give a result of one SNP but didnt give a result for another SNP. Note, these SNPs are performed at the same plate (same condition). Depending on the...
26 March 2024 7,013 0 View
Dear colleagues I am organizing tension tests on some existing steel elements (e.g., S275). I will extract the yielding strength and ultimate strength of the existing steel elements. I believe...
13 March 2024 2,035 0 View
We have this machine and the temperatures are not correct. Does anyone know how to calibrate it? Thank you in advance
05 March 2024 5,895 2 View
Question about how and types of methods to optimize steel structures for seismic resistance
29 February 2024 7,569 4 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View