Mohsen Navari

12 Questions 9 Answers 0 Followers

Questions related from Mohsen Navari

How long does e.coli survive on filter paper at room temperature?

I have been sent e.coli strains on filter paper and after 10 days I have not received them yet. I am wondering how long would the bacteria survive. Thank you in advance

22 April 2024 840 0 View

How to calibrate temperature on a Peqlab Primus 25?

We have this machine and the temperatures are not correct. Does anyone know how to calibrate it? Thank you in advance

06 March 2024 5,845 2 View

Avoid bacterial suspension aggregation?

Hi, I have a bacterial suspension which aggregates upon storage in refrigerator. I am wondering how I could avoid this aggregation. Thanks in advance

03 October 2023 1,378 0 View

OD600 conversion to packed cell volume?

Hello. I have to check the packed cell volume of a bacterial suspension, but I do not have access to a Hopkins tube. Is there ay way to convert OD600 to packed cell volume? I know this might be...

26 August 2023 8,984 0 View

How to make 5M solution of betaine?

I want to prepare 5M solution of betaine monohydrate, but it is insoluble in this concentration. Interestingly, tha Sigma datasheet says: "This product is soluble in water (50 mg/ml), yielding a...

21 June 2022 2,192 0 View

Why is my Bradford reagent green?

I prepared Bradford reagent based on the following, but at the end it was green instead of brown. What could be the reason? "First, dissolve 100mg of Coomassie Brilliant Blue G- 250 in 50ml of...

11 June 2022 2,464 2 View

Do I need to dialyze my protein sample after protamine sulphate treatment?

I have used protamine sulphate to precipitate nucleic acids from my protein sample (taq DNA polymerase) as the last step of purification. I'm wondering if I need to dialyze the sample to remove...

11 June 2022 9,017 1 View

Generally speaking, how much is the antibody concentrations of conjugate, test and control line antibodies in a lateral flow assay?

I know it depends, but am still wondering, on average, how much antibody would be used per lateral flow assay. Thanks

26 August 2021 9,575 0 View

Does sds interfere with immunization?

I want to extract a protein from polyacrylamide gel to be used for immunization. I am wondering if I need to run PAGE since sds might interfere with the immunization process. Thank you

11 February 2021 8,772 3 View

Recombinant restriction enzyme: how strong should the corresponding methyltransferase expression level be?

Hello. We are planning expression of a restriction enzyme in E. coli, and wondering how much of the corresponding methyltransferase is needed to be expressed in order to protect the host genome...

24 January 2021 2,278 4 View

How to stabilizen n acetyl cysteine in solution?

Besides EDTA, what else can I use to stabilize dissolved NAC for long term storage at 4 degrees? Thank you

23 September 2020 5,936 2 View

Somple method for anthocyanin precipitation?

I'm looking for a simple method to concentrate anthocyanin extract and to me precipitation seems the easiest. Any suggestions would be appreciated.

25 May 2019 7,583 8 View