Mohsen Navari

7 Questions 8 Answers 0 Followers

Questions related from Mohsen Navari

How to make 5M solution of betaine?

I want to prepare 5M solution of betaine monohydrate, but it is insoluble in this concentration. Interestingly, tha Sigma datasheet says: "This product is soluble in water (50 mg/ml), yielding a...

21 June 2022 2,185 0 View

Why is my Bradford reagent green?

I prepared Bradford reagent based on the following, but at the end it was green instead of brown. What could be the reason? "First, dissolve 100mg of Coomassie Brilliant Blue G- 250 in 50ml of...

11 June 2022 2,449 2 View

Generally speaking, how much is the antibody concentrations of conjugate, test and control line antibodies in a lateral flow assay?

I know it depends, but am still wondering, on average, how much antibody would be used per lateral flow assay. Thanks

26 August 2021 9,564 0 View

Does sds interfere with immunization?

I want to extract a protein from polyacrylamide gel to be used for immunization. I am wondering if I need to run PAGE since sds might interfere with the immunization process. Thank you

11 February 2021 8,738 3 View

Recombinant restriction enzyme: how strong should the corresponding methyltransferase expression level be?

Hello. We are planning expression of a restriction enzyme in E. coli, and wondering how much of the corresponding methyltransferase is needed to be expressed in order to protect the host genome...

24 January 2021 2,249 4 View

How to stabilizen n acetyl cysteine in solution?

Besides EDTA, what else can I use to stabilize dissolved NAC for long term storage at 4 degrees? Thank you

23 September 2020 5,927 2 View

Somple method for anthocyanin precipitation?

I'm looking for a simple method to concentrate anthocyanin extract and to me precipitation seems the easiest. Any suggestions would be appreciated.

25 May 2019 7,572 8 View