I am doing a gene knockout. Target gene: TRIM58. TRIM58 consist of 6 Exons. I'm inserting SFFV Promotor + Turbo GFP in the middle of Exon 3 using Cas9+sgRNA RNPs (nucleofection, LONZA 4D, EO 100 pulse) and AAVs to provide template for homology directed repair.

I did confirm that the knock-IN is inserted but on Western Blot i still see strong bands.

I was told that the knock-in should prevent the TRIM58 to be expressed. So to the question: What is your opinion/experience. Should the gene fail to be expressed, or is the protein synthesized but unfunctional? I have trouble finding papers where knock-out with knock-in is descriped or experimented with.