What is the importance of create interfaces in 3D plaxis analysis?

While you analyse the stone columns in 3D plaxis, the interface should be used. What is the importance of that.

26 January 2021 2,440 4 View

I did not find the body mass index variable in the NFHS-4 Men's file. In which file is this information about men's BMI available?

In which file is this information about men's BMI available?

25 December 2019 9,929 3 View

How do we measure Shape memory effect in alloys?

We fabricated a CuAlMn alloy thriugh induction moulding. What are different ways to measure the shape memory effect of the alloy we made?

14 July 2019 3,818 2 View

Have any one meet this error when using the fs/QCA 2.5 software?

02 May 2019 6,230 8 View

How to extract keywords quickly from a text file? Can I code this using JAVA and get the best results?

- Audio of the call is converted to text - 5 words are essential from a 1000 words text file... The words imp for a policy company will be policy no. and customer name. Company: How May i help...

21 December 2017 4,955 4 View

How can we calculate iron losses accurately using JMAG?

Please throw some light on how to calculate iron losses in FEA and through Electromagnetic analysis, particularly using JMAG software.

20 December 2017 9,338 2 View

How to find transition state in ORCA using ScanTS or OptTS?

Quantum chemistry-ORCA software package. I performed a geometry scan first : ! PBE0 SVP NumFreq D3 TightSCF ! ScanTS %geom Scan B 7 27 = 2.654, 0.9, 40 end end ----> It...

26 October 2017 140 4 View

Did anyone work on S-MAC protocol for WSN in omnet++/ Castalia framework ?

Implementing S-MAC protocol in omnet++ .

06 June 2017 8,984 1 View

Citing Product Information

I am writing a Ph.D thesis and obtained information about how a product was prepared (a purified protein sample) from the vendor. The information was exchanged via email. When I asked them for a...

07 March 2017 5,705 3 View

How to test for Ca(2+) free versus saturated Calmodulin?

Hi,I was wondering if anyone would be able to recommend a simple way to test whether a any calcium binding has occurred with calmodulin (a purified protein sample purchased from calbiochem) in a...

04 January 2017 2,379 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View