How to I identify the target DNA sequence with a set of primers?

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Robert Adolf Brinzer

Use Primer BLAST or ARTEMIS to find where they are on the reference genome. Alternatively do a PCR run and send the product for sequencing. Be aware that epigenetic modifications of the template will not be carried over to the PCR products.

Sushim Kumar Gupta

Use NCBI Primer-BLAST. You can use the nr database and also specify the target organism.