How do we select the size of the aggregates that are uses in the stone column in PLAXIS? Is there any option in PLAXIS 2D OR 3D?

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Loading control for western blot to detect Histone H1.2?

Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...

10 July 2024 9,165 1 View

How to dissolve anhydrous IrCl3 in water?

I want to prepare an aqueous solution of Ircl3. Anhydrous IrCl3 is not soluble in water. Is there any other way? Like to convert IrCl3 to IrCl3.3H2O?

20 April 2023 2,381 0 View

How to interpret the LC-MS data manually?

To identify the phytochemicals, present in the LC-MS data of a plant extract, what are the step wise procedure to be followed.

30 March 2023 7,456 1 View

How to give time series spatial data (temperature) in NetCDF format to CNN model in machine learning?

Hello everyone; I want to use CNN for downscaling, but I cannot find out how to give NetCDF data in the exact format to CNN. I really appreciate any help you can provide.

08 January 2023 5,004 2 View

Can I use anti fungal agents in MRS agar for culturing Lactobacillus?

Can I use anti fungal agents in MRS agar for culturing Lactobacillus? Whether these anti fungal agents wll affect the growth or gene expression of the bacteria or not?

18 August 2022 7,624 1 View

How one should know the boundary conditions in the Plaxis 3D program for stone columns analysis?

Please explain the boundary conditions for pile foundation analysis in PLAXIS 3D program

21 September 2021 1,500 0 View

What are the best simulation softwares used for Resin transfer molding(RTM) for manufacturing of composites?

There are so many simulation softwares available for RTM and VARTM process modeling. I wanted to know and decide which of them is preferable for modeling complex problems of flow of resin through...

10 September 2021 9,836 1 View

How to isolate Testicular Germ Cell (TGC) from mice testis ?

Dear all, I wanted to extract Testicular Germ cells from mice testis using percoll gradient ( or any other updated) protocol. But I am not finding the centrifugation speed and time. Kindly update...

05 August 2021 6,905 3 View

What is the importance of create interfaces in 3D plaxis analysis?

While you analyse the stone columns in 3D plaxis, the interface should be used. What is the importance of that.

26 January 2021 2,860 4 View

I want to replace USGS's LULC data by Bhuvan's AWiFS data, in which folder of ''geog'' do I need to replace ?

I am doing wrf simulation to study is there any impact of LULC on extreme precipitation. So I need to use Bhuvan's latest LULC data.

07 September 2020 8,763 14 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

What are the shear and normal stiffness values of an LLDPE liner in 3D numerical modeling of a stockpile?

I am seeking experimental or applicable data for the liner (LLDPE) interface in FLAC3D numerical modeling of a large stockpile. Could you please recommend suitable references? The preferred data...

05 August 2024 3,665 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View