Sudheer sr

4 Questions 7 Answers 0 Followers

Questions related from Sudheer sr

Is usage of SOD2 appropriate as oxidative stress marker ?

Hello, I am working on oxidative stress induction in cardiomyocytes using H2O2. I am using SOD2 as a marker for oxidative stress. I am going to treat H2O2 (150μM) for 12 hrs. Is using SOD2 as a...

17 November 2014 2,492 6 View

What dilution medium can I use for H2O2 for the treatment of cardiomyocytes ?

Hello, I am working on inducing oxidative stress in cardiomyocytes using H2O2. I need to dilute the H2O2 stock to my required concentration. I use DMEM as the medium for cell culture. Do I need to...

03 November 2014 2,373 6 View

Gene expression?

Can anyone suggest me how to study the expression level of genes in a tissue when I need to compare among those genes? Eg., I need to study three different kinases in a tissue and want to know...

06 August 2014 6,216 4 View

How to find DNA binding proteins using in silico tools?

I am working on a eukaryotic gene which is downregulated in pathological conditions at both DNA and protein levels. I studied the 5' region of the gene to find the promoter binding and repressor...

27 October 2013 5,515 5 View