I measured the gene expression of specific gene by qPCR, but it was undetermined. Can I write in the manuscript that it is undetermined?
Can you explain what you mean by undetermined? Nothing fluoresced? The results were ambiguous?
I agree with David
No Mai you cannot; simply because it provides no detail. Did the experiment work but result was negative ? Did the assay based on controls not work ?
In essence, you need to either get the assay working and demonstrate a real result - positive or negative - or simply say nothing at all
Unfortunately, words like 'undermined' are meaningless in a scientific context
If you can provide more detail of what you did; the specifics of how you did it and what scientifically you found perhaps I can (with others) assist you to solve your problem
Whether a thesis or paper you need to demonstrate that you have carried out an SOP accurately and adhered to good laboratory practice and even if you do not get the result you want that is perfectly acceptable; simply because it is real
Good luck and let me know If I can assist you further (by providing scientific details)
I think a good start for learning how to present expression data is this paper:
The MIQE Guidelines:Minimum Information for Publication of Quantitative
Real-Time PCR Experiments
There you will find how you need to present your qPCR data
Perhaps this paper http://www.nature.com/nprot/journal/v3/n6/full/nprot.2008.73.html you may check out, too. There are some helpful hints for earlier steps even before running PCR plus examples how to present the data. Good luck.
Sorry for not replying before, I did not get notifnotification that anyone answered my question. Thank you for your replies.
When I used the threshold used with other genes expression, there is no result with this gene, so I considered it undetectable (not undetermined as I mentioned above).
- Thresholds are a function in part of cDNA quality or quantity of particular cDNA samples/RT reactions/RNA prepsand thus might not always apply between different preps
- As long as you have exponential amplification you can determine threshold by determining act value at base of where amplification starts
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