6 Questions 13 Answers 0 Followers
Questions related from Mai M Abdelmoaty
I made transfection of some cell lines using siRNA and cationic polymer, I am sure the polyplexes were formed (NP ratio of polyplexes were determined by the agarose gel) but the transfection...
08 August 2019 9,976 5 View
I would like to extract the proteins from adherent cells to be used in ELISA assay for the detection of a nuclear protein (CDK6), I wonder which protein extraction method is the best? Because I...
17 September 2018 8,324 5 View
I have a specific DNA sequence which I could determine its location in a specific gene in the human genome. I wonder how can I know if it is located in a SNP region or no? And if it is in intron...
04 May 2018 4,831 4 View
How can I know the SNPs of a specific gene (I determine gene mRNA on NCBI then choose SNP option, is this a correct method)? How can I get graphics with high resolution of gene sequences and their...
04 April 2018 2,461 3 View
I measured the gene expression of specific gene by qPCR, but it was undetermined. Can I write in the manuscript that it is undetermined?
02 March 2016 7,056 6 View
When I make NCBI blast (nucleotide sequence) and write rats in the organism, I find the ident and query cover 100% for the gene that I made primer design for it, but also I find ident 100% and...
07 October 2014 1,761 6 View
