What is the ethical procedure to collect samples from human such as blood, tissue, sperm for genome sequencing?

Hello All Can you share your knowledge, the ethical issues for collecting the sample from the human ( Blood, tissue, sperm)

04 May 2018 6,372 0 View

Is any sequences of Viral lncRNA reported?

Hello I want reported sequences of viral non coding RNA of any virus. Is there any database or literature available so far.

03 April 2018 1,644 1 View

Coding (lnc RNA) potential of viruses?

How viruses transferring their genetic information gene to protein and how it produce lnc RNA to suppress or activate a specific genes in the host system. What are all the major difference of...

02 March 2018 2,314 0 View

How to compare time series data of gene expression studies?

Hello All I have a time series micro-array data, the format look likes, patient samples collected from Day 1 to Day 40 ( sample size 40)and control sample ( 20). I would like compare all the...

11 December 2017 2,559 3 View

How NGS applied in the field of virology? What are all the merits and demerits?

08 September 2017 1,031 0 View

How to analyze large samples using cytoscape(MCODE)?

05 June 2017 8,371 2 View

Comparison of microarray data of Human and NHP is reliable?

11 December 2016 141 0 View

Data mining applications in viral microarray datasets?

Hello every one, I looking for the application of Data mining concepts in Viral micro-array data sets.can you suggest any paper dealing with above mentioned.( not looking for paper related virus...

04 May 2016 1,370 0 View

Pathway analysis of miRNA?

03 April 2016 7,482 1 View

Meta analysis of microarray data merits and demerits?

Hello , Meta analysis of micro-array data may help to identify novel insights of gene expression data sets.Most of the meta analysis work form cancer studies. can i use this methodology for viral...

02 March 2016 4,192 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View