Hi
How do we understand the amount of gene expression level and biological impact
for ex: some gene respond to the viral infection with only minor changes that still biologically relevant vice versa dramatic changes may be biologically irrelevant.
Hello All Can you share your knowledge, the ethical issues for collecting the sample from the human ( Blood, tissue, sperm)
04 May 2018 6,474 0 View
Hello I want reported sequences of viral non coding RNA of any virus. Is there any database or literature available so far.
03 April 2018 1,757 1 View
How viruses transferring their genetic information gene to protein and how it produce lnc RNA to suppress or activate a specific genes in the host system. What are all the major difference of...
02 March 2018 2,444 0 View
Hello All I have a time series micro-array data, the format look likes, patient samples collected from Day 1 to Day 40 ( sample size 40)and control sample ( 20). I would like compare all the...
11 December 2017 2,667 3 View
Hello experts This is very common question Next generation sequencing now applied almost all biological field in including virology, the data publically available like WGS of virus and viral...
08 September 2017 1,161 0 View
Hello I am doing gene network analysis using micro array data set for identifying the gene enrichment,pathway and transcription factor analysis. My data set contain 79 sample with different time...
05 June 2017 8,491 2 View
hi to all can you give me your suggestion , Is the Human and Non human primates microarray data is reliable to compare in the context to understand the difference in gene network modules
11 December 2016 257 0 View
Hello every one, I looking for the application of Data mining concepts in Viral micro-array data sets.can you suggest any paper dealing with above mentioned.( not looking for paper related virus...
04 May 2016 1,486 0 View
Hello I want to do pathway analysis of list of miRNA which are related to multi drug resistance, can you Share any ideas about the suitable tools?
03 April 2016 7,593 1 View
Hello , Meta analysis of micro-array data may help to identify novel insights of gene expression data sets.Most of the meta analysis work form cancer studies. can i use this methodology for viral...
02 March 2016 4,339 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View