How relevant it is to carry out CRISPR/Cas based genome editing of cds obtained via De novo transcriptome sequencing? How important it is to have the genome sequence available for gene editing? What could be the possible outcomes and consequences ?
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Alka Jangra
Frederic Lepretre
Hi
genome editing means that you'll target precise sequences and in view of the editing possible errors due to mismatch priming, the best way to run this protocol is to get the entire sequence to avoid off targets multiplication.
Article Off-target effects in CRISPR/Cas9-mediated genome engineering
improvments have been done but this point still exists.
all the best
fred
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