In terms of camera based fluorescence microscopy, what does time interval mean?

I am getting slightly confused here... When the term 'time interval or delta t' is used, does it explicitly imply time between successive frames or can it also refer to the time taken to capture...

19 October 2020 3,105 5 View

Does fluorescence based imaging improve spatial resolution?

I understand specific techniques based on fluorescence and other methods can enhance the spatial resolution beyond the diffraction limit of lambda/2NA (xy-plane) in what we call as the...

03 September 2020 8,786 3 View

We are working on insect cell culture using Kimura's modified media. We have observed that cell are live for near about 20 days but no growth was obs?

We have tried different pH and substrates but still no cell division was observed.

21 July 2019 2,817 2 View

Relative gene expression analysis by 2deltaCT method?

Hello, In my experiments I divided mice into 4 groups. one group fed on control diet, second group on high fat diet (hfd), third on hfd+treatment1 and fourth on hfd+treatment2. While analysing...

27 December 2018 1,455 3 View

Is the CMOS compatibility is needed for solar cell manufacturing ??

texturing of silicon preferred mostly TMAH because of its CMOS compatibity whereever being low cost KOH and NAOH is not preferred because of its heavy mobile ion contamination. is in what sense...

20 December 2018 6,057 6 View

Research vs structural consultancy...which one should be considered for future and personal development??

Life is stuck in between consultancy and research work. Consultancy gives you practical experience along with money, however, research is limited to citations, projects etc. I know both fields are...

18 October 2018 7,373 3 View

What is the difference between the various homologs of a single coenzyme?

I was looking for CoQ10 ELISA kits and I came across some labelled as 'CoQ10 binding protein homolog A, mitochondrial' or CoQ10... homolog B..' what is the difference between these two in terms...

15 April 2018 268 4 View

How should I simulate accelerometer in comsol?

I am simulating a mems accelerometer L type spring . during eigenfreq. measurement displacement found is in order of 10^6 and it was supposed to be in micron. so is there any mistake that I am...

15 March 2017 1,757 6 View

How to dispose off the plants used in phytoremediation processes?

copper contaminated soil

11 January 2017 7,627 12 View

Can anyone recommend a good textbook on smart materials for biomedical applications?

18 December 2016 8,651 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...

09 February 2021 9,012 3 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

P53 detection in methylated and non methylated samples?

Hello everyone. I am about to search for the expression of genes including p53 in methylated and non methylated HPV samples. Should I extract DNA from the methylated samples and use SYBR green...

04 February 2021 2,749 3 View