You’re looking for Gateway-compatible binary vectors featuring an N‑terminal EYFP/GFP tag, suitable for rice transformation and gene overexpression under the CaMV 35S or maize ubiquitin (UBQ) promoters. Here are two well-established vector systems that perfectly match your needs:

1. pPUG Series (UBQ promoter + GAP‑based Gateway vectors)

From Tsuda et al. (2022): These are Gateway-based binary vectors tailored for rice, using the maize ubiquitin promoter (proUBQ) which is more reliable than 35S in rice, especially after regeneration. PMC

• pPUG2: UBQ promoter → N‑terminal GFP tag → Gateway cassette → Ideal for overexpression of N‑GFP fusion proteins. PMC
• pPUG3: UBQ promoter → Gateway cassette → C‑terminal GFP tag (for comparison/reference, not N‑terminal). PMC

Also available are pPUG1 (no tag), and mCherry equivalents (pPUG4 / pPUG5), but for GFP at the N‑terminus, pPUG2 is your go-to.

These vectors were validated for stable expression and subcellular localization in rice tissues—with UBQ ensuring strong signal maintenance across tissues. PMC

2. pRHVnGFP (Ubi promoter, N‑terminal EGFP, Gateway entry) – from pCAMBIA‑derived system

From Komori et al. et al. (via Zhang et al. toolkit): These are rice-specific Gateway-compatible binary expression vectors built on pCAMBIA backbones.

• Example vector: pRHVnGFP Ubi promoter → N‑terminal EGFP tag → Gateway acceptor site → Nos terminator Contains selection markers (e.g., hygromycin resistance via hptII under 35S promoter) and T‑DNA borders for Agrobacterium transformation. PMC

These vectors, including pRHVnGFP, have been demonstrated to produce strong GFP fluorescence in stably transformed Nipponbare rice plants—proving their effectiveness. PMC

Summary Table

Vector SystemPromoterTag TypeGateway Compatible?NotespPUG2Maize UBQN‑terminal GFPYesHighly stable expression in rice PMCpRHVnGFPMaize UBQN‑terminal EGFPYesProven in stable transgenics PMC

Additional Context

• UBQ vs. 35S promoter in rice: The maize ubiquitin promoter is known for more stable, constitutive expression across rice tissues. In contrast, CaMV 35S can be silenced post‑regeneration, though it remains active in callus cells—a feature sometimes useful for genome editing workflows. PMC+1
• Gateway cloning benefits: Both systems leverage the efficiency of Gateway recombination (LR reaction) for seamless cloning of your gene of interest into vectors with N‑terminal GFP fusions.

What to Do Next

If you'd like vector maps, sequence details, or help deciding between UBQ and 35S for your specific experimental design (e.g., tissue type, developmental stage), feel free to ask—I’d be happy to assist further!