Bilal Ahmad

2 Questions 1 Answers 0 Followers

Questions related from Bilal Ahmad

Gateway vector for gene overexpression in rice?

Hi everyone, I am new to rice transformation. I was wondering if I could get the names of some Gateway-compatible vectors containing an N-terminal EYFP/GFP tag that are widely used for rice...

11 August 2025 2,873 1 View

Hi Everyone, I am interested in fusing 3 copies of EYFP with protein of interest. I have already one copy there and want to add two more?

I have been using the NEB Hifi Assembly master mix and following the same protocol. I used three different constructs but the same vector. Whenever I do the colony PCR for screening, I always get...

31 March 2020 7,955 3 View