Can anyone elaborate how the CAAG promoter functions differently than the usual CAG promoter used in various scAAV vectors.
I am open for a good discussion.
Hi, I am facing a temperature convergence issue in Silvaco Atlas. At the threshold voltage the temperature is not converging. I applied given models models consrh bgn FLDMOB HCTE MASETTI...
02 May 2024 3,059 0 View
Hi, I'm currently trying to generate a one-vector plasmid system for a knockout using CRISPR/Cas9. I'm using lentiCRISPRv2 (ampicillin-resistant) and following the GeCKO Target Guide Sequence...
29 April 2024 3,660 0 View
History of what lead to this thinking Current issues and debates around this paradigm
01 April 2024 459 0 View
In my ddPCR assay to check my specific edits the PCR amplicon length is 100 bp, and two probes are within the amplicon. Probe 1 (labelled with FAM) is specific to mutant and Probe 2 (labelled with...
14 March 2024 7,010 1 View
I want to examine the correlation between signal intensity of Hoffa's fat pad and KL grades of knee osteoarthritis. But i am not able to get how to measure the signal intensity. Elliptical/round...
11 March 2024 9,693 1 View
If any respected member want to share the work with me.. i am ready.
20 February 2024 4,189 8 View
Hello, Is there any alternative (cheaper) Bio-Rad AutoDG-compatible tips? The price for a pack of 20 boxes has become double in 2024. Thanks
12 February 2024 1,350 0 View
Need guidance and help in understanding and interpretation of some data regarding the components forces that we get from the dynamometer after the side milling (up and down) operation. After the...
06 February 2024 4,554 5 View
WHEN WE DONE THE ELEMENTAL ANALYSIS OF THE BLACK SPOT AREA THE CHROMIUM CONCENTRATION INCREASES. CAN ANYONE PLEASE TELL ME WHAT IS THE REASON BEHIEND THE INCREASE IN THE CHROMIUM CONCENTRATION...
02 February 2024 7,918 2 View
Hi, I am currently using Bio-Rad Automated Droplet generator. I am happy with the result. I get on average 19K to 21K droplets per reaction. However, I have also encountered 1 to 2 samples...
12 December 2023 9,091 3 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
02 July 2024 5,448 2 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...
25 June 2024 1,197 0 View
I am working on a project that involves analyzing remote sensing (RS) images. I need to convert these images into vector data for further analysis. I am looking for automated methods or software...
22 June 2024 6,165 2 View
In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...
19 June 2024 7,822 3 View
Should we use special binary vectors when using plasmid rescue method to detect transgene integration site in plant? that is, there is often no ori site between LB and RB region of simple binary...
28 May 2024 1,873 0 View