9 Questions 12 Answers 0 Followers
Questions related from Pranav Seth
What impact do different promoters such as CMV, CAG, CAAG, etc., have on the efficiency of viral transductions? My observations are, NOT THAT MUCH! Any inputs?
08 August 2022 1,168 4 View
I have a vector that expresses tdTomato and i have a RFP antibody. Would in principle i get a staining. I think i should since it cannot be that different , they have similar spectrum. Thanks
23 June 2020 3,507 0 View
Can the method of electroporation be coupled with viral vector gene transfers? I haven't come across any papers that talk about it? Kindly suggest if anyone is aware of anything helpful.
15 November 2018 6,179 7 View
I am using a commercially available viral vector AAV-DJ (Capsid from AAV-DJ and ITR from serotype 2) with a transgene for eGFP. My vector has its own promoter CAG (Chicken -beta-actin hybrid) The...
22 November 2017 6,335 2 View
Does anyone know of any experience or a protocol with such a thing.
17 November 2017 4,490 4 View
It is an AAV vector
28 September 2017 3,231 3 View
To optimise subretinal eye injections .
13 July 2017 5,196 1 View
Can anyone elaborate how the CAAG promoter functions differently than the usual CAG promoter used in various scAAV vectors. I am open for a good discussion.
01 January 1970 6,736 0 View
How long does it take for a high titre AAV to start expressing in HEK293 cell line? Mine hasn't shown any expression even after 48hrs.
01 January 1970 4,933 5 View
