Question based on Integral Transform (from the book "Mathematical Methods for Physics and Engineering")?

I came across this question (attached below). I tried to solve it but got stuck in the portion where we need to calculate the inverse tranform. I found a solution of this question (also attached...

28 November 2020 7,789 3 View

What is the temperature pressure profile for a methanol water mixture?

I have a methanol-water mixture and I want to know how temperature changes pressure with varying methanol-water percentage (v/v)?

23 November 2020 1,585 1 View

Problem facing in creating custom NN script?

Dear all I have created a custom script for Neural Network for my dataset with 36data as training set and 6data as test set. My activation functions for both hidden layer and output layer is taken...

29 August 2020 975 5 View

How to create Combined graph and which software will be best for it?

Hello everyone, i want to create the combined graph with same x axis and two different y axes, one above other in same figure as shown in attachment for academic purpose. The attached file is...

17 March 2020 9,887 3 View

Problem in use of /INPUT in ANSYS APDL?

10 November 2019 8,140 3 View

How I should prepare the sample (plant extract) for FTIR?

The plant methanol extract is oily and sticky. Its not giving good result in FTIR analysis. But the extract is fresh.

12 August 2018 4,167 5 View

What is the most efficient and faster way to generate a stable cell line starting from a plasmid?

I use lipofectamine 2000 to transiently transfect my plasmid into the HeLa cells and then use selection antibiotic to make stable cell. This method does not give very good transfection efficiency...

27 July 2017 3,857 1 View

Can any one tell me what is the half life of CCCP (Carbonyl cyanide m-chlorophenyl hydrazone)

24 June 2017 3,797 2 View

Is inverse PCR the only option to mutate one central base of a plasmid?

I have a plasmid which differs by one base from wild type. This base will change the protein by one amino acid, hence I need to mutate that base which is residing in the central part of the...

06 March 2017 1,773 3 View

Ideal colony size during plasmid transormation in BL21 (DE3) cells?

When I am transforming a plasmid in BL21 (DE3) cells, plate is found to contain colonies of different size. Roughly they may be classified as small, medium and large. Are they all equivalent for...

25 January 2017 2,804 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Negative result on natural product sample cholinergic assay?

i have been doing cholinergic assay on natural product and for some of the samples, i get negative value for the inhibition activity. can anyone help me explain the meaning of the negative value...

17 February 2021 4,118 1 View