From where I can purchase Cutaneous Squamous Cell Carcinoma (SCC) cell lines, SSC12 and SSC13?

More Shahab Uddin, Ph.D's questions See All

Can a photocatalytic degradation of methylene blue from red mud be pseudo- zero order kinetics?

My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?

29 July 2024 7,404 1 View

How to calculate pseudo order kinetics?

I know the rate law. But for my photo-catalytic experiments, first 90min is adsorption (dark) and next 150min is under UV light (total 240min). How to remove the adsorption part for calculation?...

21 July 2024 8,807 6 View

Cp of Turbine is ever increasing with Tip Speed Ratio. What Could be the reason??

I am a student of Mechanical Engineering. I am doing CFD opf Archimedes Winf Turbine. I am trying to validate the result of Paper. But the problem is that tha value of Power Coefficient is ever...

05 July 2024 6,839 2 View

How to make 100ppm 1Liter methylene blue stock solution? How much weigh the dye require?

methylene blue stock solution

19 June 2024 10,030 3 View

How to weight this chemicals to mix up to make zeolite 13X-Tio2 with Fe doped?

I use molecular sieve 13X and Iron nitrate powder Fe(NO 3)3 • 9H2O. and commercial TiO2. I need to prepare 8% Fe, 8% TiO2 containing with zeolite 13X. I want to use this method below: WET...

17 June 2024 4,153 2 View

How to calculate 1milimoles of H2O2 30%, ?

for photo phenton reaction, dye concentration 10ppm, catalyst 100mg/L.

16 April 2024 1,686 4 View

Is there any application, software or website that i can use for generating plagiarism reports, against my research, without involving a university?

In case one is not registered with a university?

25 March 2024 6,126 1 View

Any plastic recycling company pestel analysis?

any plastic recycling company pestel analysis

21 March 2024 5,391 0 View

How to detect noise sources from a home appliance?

for example: I am using a refrigerator for 3 years , recenly, 2/3 months ago the noise level seem increased. I made a complain to the refrigertor manufacturing brand. Now, how the R&D team of...

27 February 2024 6,870 2 View

My catalyst Ti contaning zeolite from red mud shows different degradation at different pH.?

For pH 5 & 9 almost same time 4hr for 95%, for pH 11 less time 3hr. 100% for pH 3 . 4hr, 75% Why this abnormal happened?

20 February 2024 6,525 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Shahab Uddin, Ph.D

Thank you so much

Abdulhadi Alamodi

Dr. Shahab

if you like I can send you some samples . You don’t need to buy or purchase that.

are you working in same area abdulhadi? Please share what are cell line you have it? That will be great

Mohamed Hassan and I had worked previously on melanoma projects before. We can manage both tongue and cutaneous.

Imteyaz Qamar

You may get from ATCC

Ssc12 and Ssc13 are not available in ATCC

Then you may have to request someone. I hope you will get