How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View

How to increase mesh quality of a cfd model in ansys fluent?

How can one improve mesh skewness, orthogonality, aspect ratio?

28 February 2021 7,730 3 View

Why is my CFD problem (mini heat sink rectangular channel) not converging while having good quality mesh?

My CFD problem is having two interconnected rectangular channel, which is known as mini heat sink. I have already performed parallel flow inside of an interconnected mini heat sink. However, I...

19 February 2021 3,209 3 View

Should we analyze or relinquish the clinically confirmed data of COVID-19?

Hello everybody As you know, due to the high cost and time-consuming of laboratory assays, like PCR, for the suspected cases of COVID-19, doing this type of investigation is not possible for all...

06 February 2021 6,122 3 View

I get tumor tissue from the patients after the surgery. How to isolate the top layer of cells from a tumor?

I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.

25 January 2021 6,663 1 View

Can anybody help to find a tool for assessing study habits of students?

It is for my research purpose

21 December 2020 833 3 View

Polyelectrolyte complex ion pairing through IR?

Is it possible to observe ion pairing (contact ion or solvent separated ion) of polyelectrolyte complexes through liquid FTIR? Although the PECs have been widely studied in the last two decades, I...

12 November 2020 4,800 1 View

How can I get better picture for the attached TEM Image??

I used TEM equipped with tungsten gun to obtain attached image (several other too, like shown). Side blotting technique followed by negative staining (1% PTA for 30 sec) was used. I cannot get the...

31 October 2020 1,902 5 View

How can I get Nusselt number for mini rectangular channels using fluent?

I am validating my CFD mini rectangular channel model. For that purpose, I need to know how to get the Nu number accurately. For Calculating Nusselt no. from fluent post-processing as follows:...

24 October 2020 3,444 5 View

What is the possible biological explanation of the mutational pressure as a driving force on the evolution of particular species ?

In one analysis major role of mutation pressure was observed in codon usage bias of some species

19 October 2020 4,755 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View