Can crude enzyme be used for immobiliazation?

Can crude enzyme be utilized as immobilized enzyme for industrial application if the crude enzyme shows high activity?

13 July 2020 8,672 4 View

How can I overlay two raster without turning them in vector format?

I have two raster one is elevation class map from DEM reclassification and another is snow cover fraction map. I need to calculate elevation class wise Snow cover fraction(%). I do not want to...

27 January 2020 730 5 View

How to conjugate a dye to the N-terminus of poly-glutamic acid peptide?

Hi! I am trying to conjugate a dye in its NHS ester form to the N-terminus of poly-glutamic acid peptide ( which is already available in the form of a white solid). I tried 3 times and am tring...

27 June 2019 8,798 3 View

Is it necessary to purify PCR products before cycle sequencing?

I will purify the cycle sequencing products using EDTA/EtOH precipitation method before loading to 96 well plate.

30 November 2018 6,932 4 View

In case of Box behnken design reduced quartic model, do I need to mention all the insignificant terms in the ANNOVA table and polynomial equation?

When I try to reduce those terms the R2 value reduces. As a result I have many terms whose sum of squares and df values are 0. The p value and all other values fall in range.

22 May 2018 8,088 4 View

How to measure voltage dependent strain of a thin film without using electric field dependent XRD???

because we have not any facility of electric field dependent XRD but all the papers suggested this electric field dependent XRD. If anyone can explore any idea then please help me

15 April 2018 263 2 View

In an XRD data if the peak are same with ICSD data but intensity is not matched with ICSD data then is anything worng???

My XRD data for NdNiO3 is similar with ICSD data but intensity are not matching with ICSD data

11 March 2018 8,823 4 View

How a core flood experiment for conformance control can be designed?

The limitation we are currently having is that cores of length upto 6 inch can only be used in our system and we can not prepare composite cores.

03 September 2017 9,093 2 View

Can zero field cooled MH loop measured after some times (few months) for same magnetic sample at same temperature be different?

17 February 2017 6,694 5 View

PLD Vs RF sputtering?

How PLD and RF sputtering are different over each other ? Or why one should choose PLD over sputtering except depositing complicated oxides (e.g: super conductors or perovskites). It is well known...

12 October 2016 1,464 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View