Adding trizol directly into cell culture flask will damage the cells and what is the other alternative to add trizol into cells?
You can add trizol directly to the cells and then scrape to finally collect cells and continue with the RNA extraction.
Hi poonam. I work with adherent cells and i lyse my cells directly in a 6 well plate with trizol, never had any issues With my results. You can trypsinise first if you wish, perhaps it is easier to lyse a pellet in your case, rather than scraping a flask as it it less handy in my opinion. But depending on your cells, specially stem cells, the less trypsine you use, the better! You can also grow your cells in petri dishes with similar growth area as a t25 flask and make it easier! Hope this helps :)
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