Poonam Bhagriya

4 Questions 1 Answers 0 Followers

Questions related from Poonam Bhagriya

For RNA isolation ,adding trizol directly into the culture flask (T25) will harm the cells?

Adding trizol directly into cell culture flask will damage the cells and what is the other alternative to add trizol into cells?

21 November 2019 5,406 2 View

Change in E3 media pH from 7.2 to 7.8 to dissolve methotraxate will harm the embryo?

Will there be any neuronal defect or increase in the death of Zebrafish embryo if the pH of E3 media is changed from 7.2 to 7.8 for dissolving the methotrexate.

19 November 2019 1,546 0 View

Why A549 Cell line morphology is changed or why cells are looking unhealthy?

After trypsnisation cells morphology is changed within 24 hours,media was changed after cells are attached but still cells are looking unhealthy why?

18 November 2019 4,452 4 View

Cryopreservation of zebrafish embryo ?

Hello everyone, I need to preserve zebrafish embryo for whole mount immunostaining. Can anyone suggest me in which solution should I need to cryopreserve the pre-hatched zebrafish embryo ? Thanks.

08 April 2019 1,239 0 View