I regularly do western blotting with total cell lysates from breast cancer cell lines. Many times I struggle for equal loading of the samples in gels. Generally I use Bradford reagent to quantitate amount of proteins in my samples. Can anybody suggest which is the most sensitive and accurate method for quantification of proteins in total cell lysates?
Thank you so much Kou for nice suggestion, since our lab is concerned with biological assays for cancer, any other alternative technique like any kit or reagent based analysis would be easier and feasible for us. Is there any such kind of alternatives available for more accurate quantification of protein in samples for western blotting?
Thanks @Kou Hayakawa for your response. We do have HPLC and other sophisticated analytical facilities in our Institution. But I wonder how the cost of HPLC (Instrumentation, solvent and other) is less than Western blotting (SDS-PAGE). However, I just wanted to know alternatives for quantification of protein which is required for Western blotting (Signalling pathway elucidation in cancer biology).
Thanks...
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