Can any one send me some articles related to the role of MPS1 kinase in CHK2 actvation?

07 August 2016 4,368 0 View

What is appropriate incubation time for primary antibody in western blot analysis?

Primary antibodies, Incubation time, western blotting, ELISA, SDS-PAGE

07 August 2016 6,517 22 View

Can blotting effect if some amount of SDS-Buffer present in transfer buffer solution?

Can blotting effect if some amount of SDS-Buffer present in transfer buffer solution?

06 July 2016 1,066 5 View

I am using BSA for blocking membrane for Phosphospecific antibodies but getting much background, could any one help me how to overcome this problem?

I am using BSA for blocking membrane for Phosphospecific antibodies but getting much background, could any one help me how to overcome this problem?

06 July 2016 2,109 4 View

I am working on CHK2 kinase protein and every times after western blot getting blackground stain also, washed 3 times10 minutes each,what is problem ?

western blotting, SDS-PAGE, CHK2, CHK1, Washing buffer, PBS, TBS

06 July 2016 9,108 8 View

For phosphospecific antibodies why milk blocking buffer is not good?

06 July 2016 5,507 1 View

Effect of ethanol on MCF-7 breast cancer cell line?

03 April 2016 5,196 2 View

Can any one please send me rad 9b gene related research article??

02 March 2016 3,858 0 View

What is main difference between Ct and Cq value? When do we use Ct value and when Cq In Real Time PCR?

11 December 2015 612 13 View

Which one is better for lower Ct value in rt-PCR either Taqman's OR SYBER Green?

I am doing analysis of CML cell tramscript expression by rtPCR data

11 December 2015 137 6 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View