Yogendra Verma

13 Questions 15 Answers 0 Followers

Questions related from Yogendra Verma

What is appropriate incubation time for primary antibody in western blot analysis?

Primary antibodies, Incubation time, western blotting, ELISA, SDS-PAGE

08 August 2016 6,548 22 View

Can any one send me some articles related to the role of MPS1 kinase in CHK2 actvation?

Cancer, cell cycle biology, protein chemistry, cell cycle checkpoint, DNA regulatory protein, western blot, SDS-PAGE, DNA repair protein

08 August 2016 4,404 0 View

I am working on CHK2 kinase protein and every times after western blot getting blackground stain also, washed 3 times10 minutes each,what is problem ?

western blotting, SDS-PAGE, CHK2, CHK1, Washing buffer, PBS, TBS

07 July 2016 9,145 8 View

For phosphospecific kinase proetin how much time incubation (45') for secondary antibody is good for perfect result and washing time after incubation?

western BLOTTING, SECONDARY ANTIBODIES, PBS, Incubation time, SDS-PAGE

07 July 2016 9,583 2 View

For phosphospecific antibodies why milk blocking buffer is not good?

Phosphospecific antibodies, western blotting, washing buffer, blocking buffer,

07 July 2016 5,539 1 View

Can blotting effect if some amount of SDS-Buffer present in transfer buffer solution?

07 July 2016 1,100 5 View

I am using BSA for blocking membrane for Phosphospecific antibodies but getting much background, could any one help me how to overcome this problem?

07 July 2016 2,143 4 View

Effect of ethanol on MCF-7 breast cancer cell line?

Ethanol, MCF 7 cell line, Breast cancer cell, SDS-PAGE, Western Blot, RO60,

04 April 2016 5,225 2 View

Can any one please send me rad 9b gene related research article??

RAD9b, msp1, CHK1, CHK2, mitosis

03 March 2016 3,894 0 View

Which one is better for lower Ct value in rt-PCR either Taqman's OR SYBER Green?

I am doing analysis of CML cell tramscript expression by rtPCR data

12 December 2015 165 6 View

What is main difference between Ct and Cq value? When do we use Ct value and when Cq In Real Time PCR?

Some time we use only Ct value and some time Cq value in Real Time-PCR gene expression studies. I am little bit confused, what is main difference between these?

12 December 2015 650 13 View

How to increase the accuracy of the Plasmid Map???

The accuracy of the Plasmid map is dependent on how precisely the exact size of a fragment is determined..

11 November 2015 7,381 0 View

How could I analyse rt-qPCR expression in between untreated and treated Tyrosin Kinase Inhibitor in chronic myeloid leukemia BCR-ABL1 gene Expression?

I am doing an experiment on Chronic myeloid Leukemia cell line K652 and facing problem in analysis of their expression on both treated with TKI and untreated sample of BCR-ABL1 gene.

11 November 2015 9,919 3 View