What is appropriate incubation time for primary antibody in western blot analysis?

Primary antibodies, Incubation time, western blotting, ELISA, SDS-PAGE

07 August 2016 6,517 22 View

For phosphospecific kinase proetin how much time incubation (45') for secondary antibody is good for perfect result and washing time after incubation?

western BLOTTING, SECONDARY ANTIBODIES, PBS, Incubation time, SDS-PAGE

06 July 2016 9,545 2 View

Can blotting effect if some amount of SDS-Buffer present in transfer buffer solution?

Can blotting effect if some amount of SDS-Buffer present in transfer buffer solution?

06 July 2016 1,066 5 View

I am using BSA for blocking membrane for Phosphospecific antibodies but getting much background, could any one help me how to overcome this problem?

I am using BSA for blocking membrane for Phosphospecific antibodies but getting much background, could any one help me how to overcome this problem?

06 July 2016 2,109 4 View

I am working on CHK2 kinase protein and every times after western blot getting blackground stain also, washed 3 times10 minutes each,what is problem ?

western blotting, SDS-PAGE, CHK2, CHK1, Washing buffer, PBS, TBS

06 July 2016 9,108 8 View

For phosphospecific antibodies why milk blocking buffer is not good?

06 July 2016 5,507 1 View

Effect of ethanol on MCF-7 breast cancer cell line?

03 April 2016 5,196 2 View

Can any one please send me rad 9b gene related research article??

02 March 2016 3,858 0 View

What is main difference between Ct and Cq value? When do we use Ct value and when Cq In Real Time PCR?

11 December 2015 612 13 View

Which one is better for lower Ct value in rt-PCR either Taqman's OR SYBER Green?

I am doing analysis of CML cell tramscript expression by rtPCR data

11 December 2015 137 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View