Cancer, cell cycle biology, protein chemistry, cell cycle checkpoint, DNA regulatory protein, western blot, SDS-PAGE, DNA repair protein
Primary antibodies, Incubation time, western blotting, ELISA, SDS-PAGE
07 August 2016 6,584 22 View
western BLOTTING, SECONDARY ANTIBODIES, PBS, Incubation time, SDS-PAGE
06 July 2016 9,610 2 View
Can blotting effect if some amount of SDS-Buffer present in transfer buffer solution?
06 July 2016 1,127 5 View
I am using BSA for blocking membrane for Phosphospecific antibodies but getting much background, could any one help me how to overcome this problem?
06 July 2016 2,167 4 View
western blotting, SDS-PAGE, CHK2, CHK1, Washing buffer, PBS, TBS
06 July 2016 9,169 8 View
Phosphospecific antibodies, western blotting, washing buffer, blocking buffer,
06 July 2016 5,568 1 View
Ethanol, MCF 7 cell line, Breast cancer cell, SDS-PAGE, Western Blot, RO60,
03 April 2016 5,244 2 View
RAD9b, msp1, CHK1, CHK2, mitosis
02 March 2016 3,920 0 View
Some time we use only Ct value and some time Cq value in Real Time-PCR gene expression studies. I am little bit confused, what is main difference between these?
11 December 2015 708 13 View
I am doing analysis of CML cell tramscript expression by rtPCR data
11 December 2015 191 6 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View