What are all the bioinformatic tools for studying functional annotations of proteins?

Hi I need some suggestions regarding the bioinformatics tools that can be used to study the functional annotations and pathways of proteins

01 February 2018 6,387 3 View

Is it possible to purify the Sulfated polysaccharides (fucoidan) through Fast protein liquid chromatography (FPLC)?

Can i purify the Fucoidan through FPLC instruments.

10 November 2016 1,631 4 View

Should Albumin be removed before 2DE gel electrophoresis from serum sample?

I am going to perform a 2DE gel electrophoresis from serum sample, before start a 2DE; will I remove albumin protein in serum sample? Is this important step? If anybody know help me.

07 August 2014 2,828 3 View

Can anybody suggest what the physical difference is between sprague dawley and wistar rats?

How can I identify the difference between the sprague dawley and wistar rats strains?

04 May 2014 5,715 1 View

Why did miRNA increase in a diseased condition?

In a cardiovascular disease condition many specific miRNA level is increased. It is used for diagnostic biomarker. What is the mechanism of increased miRNA level in heart disease?

02 March 2014 4,257 4 View

What are the possible works that can be done after proteomics?

Hi i'm working on proteomics and currently I have identified certain proteins using 2DE and LCMS and western blotting wat is the next stage of this work

01 January 1970 4,217 4 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View