I am going to perform a 2DE gel electrophoresis from serum sample, before start a 2DE; will I remove albumin protein in serum sample? Is this important step? If anybody know help me.
see this link you will get some idea
http://onlinelibrary.wiley.com/doi/10.1002/bmb.20353/pdf
It is better if you remove the albumin by TCA precipitation.
Well, if you remove the most abundant proteins of the serum (albumin and immunoglobulin) you are going to improve the resolution of the less abundant proteins because in the 2D. Just imagine a huge amount of albumin in the gel, that giant spot is going to hide some other proteins of similar molecular weight and isoelectric point, so that if you remove albumin you will be able to discover many more spots in the same area that belongs to albumin.
There are many easy to use mini chromatography spin columns to remove in few steps the most abundant proteins (albumin and immunoglobulin), so if you have the chance to use one of those with your serum samples, go ahead and do it
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