I've reached now passage number 50 and I want to extract the protein for proteomics study
Check out the July/August issue of Oncology News (http://www.oncologynews.biz/julaug13.html)
In the Editorial section Denys Wheatley states the following about cell lines in cancer research: "Subculturing of cells over long periods produces subpopulations with some altered characteristics, and thus the genealogy (and divergence) of cell lines needs consideration."
What exactly is your aim? Do you want to express a recombinant protein? Or a naturally ocurring protein? I mean, in both cases you have to figure out the best passage number for your needs. Therefore, I would extract the protein of interest from different cell batches with different passaging numbers and check these proteins carefully for any alteration.
When you are doing the feeding/splitting regularly there shouldn't be any problem with that. But since it's a cancer cell line, some subclones may arise which are genetically or even morphologically different - in that case you'd see it visually under the microscope.
I agree with the previous answer: if you really want to be sure then
"extract the protein [...] from different cell batches with different passaging numbers and check [...] for any alteration." Plus, look at the proteins that characterize the cell line.
Check out the July/August issue of Oncology News (http://www.oncologynews.biz/julaug13.html)
In the Editorial section Denys Wheatley states the following about cell lines in cancer research: "Subculturing of cells over long periods produces subpopulations with some altered characteristics, and thus the genealogy (and divergence) of cell lines needs consideration."
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