For biosensing (or ELISA), I know that Tween20 is a good detergent to use for reducing non-specific binding for primary and secondary antibodies. Would it be also useful to dilute patient serum/plasma?
Thank you!
Tween 20 has been widely used in ELISA sample dilution buffer. You can try.
We use Tween 80 in an assay buffer used to resuspend extracted samples for ELISA:
2.42g Trishydroxyaminomethane (20 mmol/L)
17.9g NaCl (0.3 mol/L)
1 g bovine serum albumin (BSA)
1 mL Tween 80
Dissolve and fill to 1 L H20, adjust to pH 7.5 (about 17 mL HCl – 1 mol/L)
I would recommend doing tests of parallelism and accuracy to make sure that however you're diluting your samples isn't interfering with the assay. Also, run your buffer straight on your plate to make sure there isn't non-specific binding coming from the buffer itself.
- Badges
- Science method
- Similar topics
- Medicine
- Immunology
- Immunology Techniques
- Immunoassay
- ELISA