How to align large plasmids (20Kb+) in Mac Vector?

I was trying to align 3 plasmids each around 20Kb in Mac Vector. I used the "Align to ref." tab followed by adding the sequences and then aligned them. However only a portion of each sequence is...

03 February 2020 2,635 1 View

How to do overlap PCR?

Hi I am trying to join two linear 1.2 kb fragments with overlaps. I read a protocol which says at least 1/2 of the PCR mix has to be the equimolar concentrations of the fragment. Why is so much...

30 May 2019 9,234 2 View

How can I apply a thermo-mechanical load in hypermesh?

I want to determine the stresses for a laminated composite plate under thermo-mechanical load.

24 May 2019 9,180 4 View

Can I get MATLAB code for vibrational analysis of composite plate?

Iam working on vibrational analysis of laminated composite plate using MATLAB.

28 October 2018 7,641 3 View

How we can determine individual sum of squares for independent variables (factors) in the central composite design?

I am trying to solve central composite design ANOVA table manually. It has total 30 number of runs which includes 16 factorial runs,8 axial runs and 6 center runs. so, far using regression method...

23 April 2018 2,103 1 View

What if Sodium pyruvate is added in excess in DMEM cell culture media?

What happens if we accidentally add more (5 ml of 200 mM) sodium pyruvate to 500 ml DMEM cell culture media that already contains 110 mg/ml sodium pyruvate in it (from company)? How will it...

29 November 2017 6,582 2 View

Why carcinoma cell lines (e.g. HepG2, Caco-2, RAW) are used in invitro cytotoxicity experiments?

HepG2 is considered as a model system for various cytotoxicity experiments according to many studies. Won't it produce wrong results if carcinoma cells have been used for such studies as such...

21 August 2017 5,310 7 View

What is the best place to find high power LEDs for GFP illumination?

I am looking for a high power LED with a central wavelength of around 488 nm. I know Thorlabs sells one at 490 nm that supplies 216 mW of optical power at 488 nm. However, I need at least 1 W at...

01 June 2017 4,234 3 View

Is there any standard criterion to select optimum number of sequence candidates for establishing a phylogeny tree in bacteria using MEGA?

for example, in case of 16s rRNA nBLAST results, I have almost 80 similar sequences with % similarity ranging from 99% and going down till 94% ?

16 March 2017 4,772 2 View

Nusselt Number corelation for hot liquid flow through inner annulus of pipe in case of Laminar flow.

I encountered a problem in my project work on heat exchangers where to find heat transfer coefficients on the defined conditions the Reynolds number came to be 1753.8 while the Prandtl number is...

08 March 2017 4,652 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View