Is there any combination of fluorochromes, in a blue & red laser cytometer (FACSVerse), that allows me to do a 3 color setup with no overlapping or little?
At the moment I am using FITC, PE and APC channels, which seems to be a pretty standard combination, but I would need to try a different one without big overlapping like the one between FITC and PE.
I would appreciate any suggestions or changes to that combination.
Sophie Lavinia Botta Gordon-Smith
You could try out AlexaFluor647,AlexaFluor700,APC-Cy7,APC-H7 I think.
Are you sure your FACSVERSE does not have a yellow-green laser?
If you have the right lasers, try some Brilliant violet (BV) colours, they are very good.
Good luck!
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