How do I transfer documents relating to case study to be reopened for the requested enquiries?

MT-pyramid Wholistic Geometric Quester: mtpyramid.weebly.com has explorations on diagnoses and treatment; The case suffered long period of repeated "mistakes"; Transformed to Wholistic Geometric...

15 March 2020 3,395 3 View

Can lysozyme can hydrolyze chitosan nanoparticles (for gene delivery) ?

I work on kind of chitosan nanoparticles(Cs-NPs) as gene delivery vectors. I want to find the ability of the plasmid-complexes in preventing DNA migration by gel electrophoresis. sorrily there is...

30 December 2017 4,002 3 View

Does any one know what the reason of a peak in melt curve after 95 degrees centegrade is?

As you see in the picture attached that there are 3 graphs that have a short peak after 90 degrees and 3 others that have rising trend. Do you know what I can do to optimize Real-Time PCR for my...

14 August 2016 4,740 3 View

Does anyone have the PDF file of Foster, S. H. (1992). The communicative competence of young children?

This is a resource book in psycholinguistics and FLA. I was wondering someone could send its pdf file to me. I cannot buy that from the publishing centers due to my countries constraints in...

15 September 2015 2,959 5 View

What is the best protocol to extract cells from urine samples?

I am supposed to do realtime PCR to investigate gene expression.

08 February 2014 5,408 10 View

HI,a good question

I have a picture , and need to find a particular part in it,like a rectangular shap. what is the best to acces it with a mlp or hopfield network.all of this must be happen in less than 1 minute...

30 December 2010 7,969 0 View

How to do the refinement of a new material's XRD pattern?

Hi every body I have synthesized a new material in powder. Absolutely it is polycrystalline not single crystal. I am looking for a way to analyze this XRD pattern? I have read in papers about...

01 January 1970 5,599 4 View

How well do we know our-"SELF"?

How well do we know our "SELF"? Was questioned within the Network recently. There have been interesting revelation. The result has created Innovative topic workshop. How can it be organised please?

01 January 1970 7,914 17 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View