We are planning to check the level of phosphorylation of NF-KB in the CD4+ T cell population after activation with anti-CD3/CD28 and treatment for 15 minutes. However, I constantly see two fixed bands with MW around 65 and 25 KDa which might come from serum proteins but when we removed FBS from the media, they are still there. Since we're looking for p-NF-KB in 65 kDa size, how can I fix this issue?
Jeanne Pawitan
@Mehri,
The 25 KDa might be NFKB isoform due to alternative splicing. If you do not want the 25 KDa isoform, you should look for methods to prevent this alternative splicing.
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